Joint International Meeting Perspectives in Vascular Biology

Joint International Meeting Perspectives in Vascular Biology

26–28 September 2016 • Frankfurt, Germany

CRISPR/Cas9 workshop

DNA manipulation via genome editing has been a breakthrough in the scientific community enabling a better understanding of the relationship between the genome and its functions, providing remarkable insights into the center of disease and documentation of new targets for medical intervention.
Improvements in genome editing technologies have overcome many challenges and allow nowadays researchers to precisely manipulate genomes in cell lines and animals.

One of the most exciting systems for genome editing is Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein (CRISPR/Cas). With this system genes could be added, removed, and even nucleotides can be manipulated.

In this workshop, we will learn how CRISPR technology has fueled a genome editing revolution, how knockouts are performed in cell culture systems and how one can use it in practice by:
• strategies to find the appropriate target region
• searching for candidate guide RNAs (gRNAs) for Cas9 with different online tools
• minimizing off-target effects
• molecular tools to achieve a CRISPR/Cas9 mediated gene knockout
• learning how cutting-edge systems could still be improved

max. 20

2 hours

Requirements for the practical course
notebook with Wifi

included, please register here